A Versatile Tiling Light Sheet Microscope for Imaging of Cleared Tissues

A Versatile Tiling Light Sheet Microscope for Imaging of Cleared Tissues

Summary: We present a tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of cleared tissues with micron-scale (4 × 4 × 10 μm3) to submicron-scale (0.3 × 0.3 × 1 μm3) spatial resolution. The resolving ability is improved to sub-100 nm (70 × 70 ×...

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Bibliographic Details
Main Authors: Yanlu Chen, Xiaoliang Li, Dongdong Zhang, Chunhui Wang, Ruili Feng, Xuzhao Li, Yao Wen, Hao Xu, Xinyi Shirley Zhang, Xiao Yang, Yongyi Chen, Yi Feng, Bo Zhou, Bi-Chang Chen, Kai Lei, Shang Cai, Jie-Min Jia, Liang Gao
Format: Article
Language:English
Published: Elsevier 2020-11-01
Series:Cell Reports
Subjects:
light sheet microscopy
tiling light sheet microscopy
tiling light sheet selective plane illumination microscopy
tissue clearing
tissue expansion
cleared tissue imaging
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124720313383

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http://www.sciencedirect.com/science/article/pii/S2211124720313383

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