Somatic Cell Nuclear Transfer Followed by CRIPSR/Cas9 Microinjection Results in Highly Efficient Genome Editing in Cloned Pigs

Somatic Cell Nuclear Transfer Followed by CRIPSR/Cas9 Microinjection Results in Highly Efficient Genome Editing in Cloned Pigs

The domestic pig is an ideal “dual purpose” animal model for agricultural and biomedical research. With the availability of genome editing tools such as clustered regularly interspaced short palindromic repeat (CRISPR) and associated nuclease Cas9 (CRISPR/Cas9), it is now possible to perform site-sp...

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Bibliographic Details
Main Authors: Timothy P. Sheets, Chi-Hun Park, Ki-Eun Park, Anne Powell, David M. Donovan, Bhanu P. Telugu
Format: Article
Language:English
Published: MDPI AG 2016-12-01
Series:International Journal of Molecular Sciences
Subjects:
CRISPR/Cas
SCNT
pig
knockout
microinjection
Online Access:http://www.mdpi.com/1422-0067/17/12/2031

Internet

http://www.mdpi.com/1422-0067/17/12/2031

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