Real-Time In-Cell NMR Reveals the Intracellular Modulation of GTP-Bound Levels of RAS

Real-Time In-Cell NMR Reveals the Intracellular Modulation of GTP-Bound Levels of RAS

Summary: The small guanosine triphosphatase (GTPase) RAS serves as a molecular switch in signal transduction, and its mutation and aberrant activation are implicated in tumorigenesis. Here, we perform real-time, in-cell nuclear magnetic resonance (NMR) analyses of non-farnesylated RAS to measure tim...

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Bibliographic Details
Main Authors: Qingci Zhao, Ryu Fujimiya, Satoshi Kubo, Christopher B. Marshall, Mitsuhiko Ikura, Ichio Shimada, Noritaka Nishida
Format: Article
Language:English
Published: Elsevier 2020-08-01
Series:Cell Reports
Subjects:
RAS
small GTPase
oncogenic mutants
in-cell NMR
time-resolved NMR
molecular crowding
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124720310597

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http://www.sciencedirect.com/science/article/pii/S2211124720310597

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