Fluid proteomics of CSF and serum reveal important neuroinflammatory proteins in blood–brain barrier disruption and outcome prediction following severe traumatic brain injury: a prospective, observational study
Abstract Background Severe traumatic brain injury (TBI) is associated with blood–brain barrier (BBB) disruption and a subsequent neuroinflammatory process. We aimed to perform a multiplex screening of brain enriched and inflammatory proteins in blood and cerebrospinal fluid (CSF) in order to study t...
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doaj-3d84e0f8d78040568828c77636fbb4022021-03-14T12:10:34ZengBMCCritical Care1364-85352021-03-0125111810.1186/s13054-021-03503-xFluid proteomics of CSF and serum reveal important neuroinflammatory proteins in blood–brain barrier disruption and outcome prediction following severe traumatic brain injury: a prospective, observational studyCaroline Lindblad0Elisa Pin1David Just2Faiez Al Nimer3Peter Nilsson4Bo-Michael Bellander5Mikael Svensson6Fredrik Piehl7Eric Peter Thelin8Department of Clinical Neuroscience, Karolinska InstitutetDivision of Affinity Proteomics, Department of Protein Science, SciLifeLab, KTH-Royal Institute of TechnologyDivision of Affinity Proteomics, Department of Protein Science, SciLifeLab, KTH-Royal Institute of TechnologyDepartment of Clinical Neuroscience, Karolinska InstitutetDivision of Affinity Proteomics, Department of Protein Science, SciLifeLab, KTH-Royal Institute of TechnologyDepartment of Clinical Neuroscience, Karolinska InstitutetDepartment of Clinical Neuroscience, Karolinska InstitutetDepartment of Clinical Neuroscience, Karolinska InstitutetDepartment of Clinical Neuroscience, Karolinska InstitutetAbstract Background Severe traumatic brain injury (TBI) is associated with blood–brain barrier (BBB) disruption and a subsequent neuroinflammatory process. We aimed to perform a multiplex screening of brain enriched and inflammatory proteins in blood and cerebrospinal fluid (CSF) in order to study their role in BBB disruption, neuroinflammation and long-term functional outcome in TBI patients and healthy controls. Methods We conducted a prospective, observational study on 90 severe TBI patients and 15 control subjects. Clinical outcome data, Glasgow Outcome Score, was collected after 6–12 months. We utilized a suspension bead antibody array analyzed on a FlexMap 3D Luminex platform to characterize 177 unique proteins in matched CSF and serum samples. In addition, we assessed BBB disruption using the CSF-serum albumin quotient (Q A), and performed Apolipoprotein E-genotyping as the latter has been linked to BBB function in the absence of trauma. We employed pathway-, cluster-, and proportional odds regression analyses. Key findings were validated in blood samples from an independent TBI cohort. Results TBI patients had an upregulation of structural CNS and neuroinflammatory pathways in both CSF and serum. In total, 114 proteins correlated with Q A, among which the top-correlated proteins were complement proteins. A cluster analysis revealed protein levels to be strongly associated with BBB integrity, but not carriage of the Apolipoprotein E4-variant. Among cluster-derived proteins, innate immune pathways were upregulated. Forty unique proteins emanated as novel independent predictors of clinical outcome, that individually explained ~ 10% additional model variance. Among proteins significantly different between TBI patients with intact or disrupted BBB, complement C9 in CSF (p = 0.014, ΔR 2 = 7.4%) and complement factor B in serum (p = 0.003, ΔR 2 = 9.2%) were independent outcome predictors also following step-down modelling. Conclusions This represents the largest concomitant CSF and serum proteomic profiling study so far reported in TBI, providing substantial support to the notion that neuroinflammatory markers, including complement activation, predicts BBB disruption and long-term outcome. Individual proteins identified here could potentially serve to refine current biomarker modelling or represent novel treatment targets in severe TBI.https://doi.org/10.1186/s13054-021-03503-xTraumatic brain injuryProtein biomarkersProteomicsNeuroinflammationBlood–brain barrierApolipoprotein E4 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Caroline Lindblad Elisa Pin David Just Faiez Al Nimer Peter Nilsson Bo-Michael Bellander Mikael Svensson Fredrik Piehl Eric Peter Thelin |
spellingShingle |
Caroline Lindblad Elisa Pin David Just Faiez Al Nimer Peter Nilsson Bo-Michael Bellander Mikael Svensson Fredrik Piehl Eric Peter Thelin Fluid proteomics of CSF and serum reveal important neuroinflammatory proteins in blood–brain barrier disruption and outcome prediction following severe traumatic brain injury: a prospective, observational study Critical Care Traumatic brain injury Protein biomarkers Proteomics Neuroinflammation Blood–brain barrier Apolipoprotein E4 |
author_facet |
Caroline Lindblad Elisa Pin David Just Faiez Al Nimer Peter Nilsson Bo-Michael Bellander Mikael Svensson Fredrik Piehl Eric Peter Thelin |
author_sort |
Caroline Lindblad |
title |
Fluid proteomics of CSF and serum reveal important neuroinflammatory proteins in blood–brain barrier disruption and outcome prediction following severe traumatic brain injury: a prospective, observational study |
title_short |
Fluid proteomics of CSF and serum reveal important neuroinflammatory proteins in blood–brain barrier disruption and outcome prediction following severe traumatic brain injury: a prospective, observational study |
title_full |
Fluid proteomics of CSF and serum reveal important neuroinflammatory proteins in blood–brain barrier disruption and outcome prediction following severe traumatic brain injury: a prospective, observational study |
title_fullStr |
Fluid proteomics of CSF and serum reveal important neuroinflammatory proteins in blood–brain barrier disruption and outcome prediction following severe traumatic brain injury: a prospective, observational study |
title_full_unstemmed |
Fluid proteomics of CSF and serum reveal important neuroinflammatory proteins in blood–brain barrier disruption and outcome prediction following severe traumatic brain injury: a prospective, observational study |
title_sort |
fluid proteomics of csf and serum reveal important neuroinflammatory proteins in blood–brain barrier disruption and outcome prediction following severe traumatic brain injury: a prospective, observational study |
publisher |
BMC |
series |
Critical Care |
issn |
1364-8535 |
publishDate |
2021-03-01 |
description |
Abstract Background Severe traumatic brain injury (TBI) is associated with blood–brain barrier (BBB) disruption and a subsequent neuroinflammatory process. We aimed to perform a multiplex screening of brain enriched and inflammatory proteins in blood and cerebrospinal fluid (CSF) in order to study their role in BBB disruption, neuroinflammation and long-term functional outcome in TBI patients and healthy controls. Methods We conducted a prospective, observational study on 90 severe TBI patients and 15 control subjects. Clinical outcome data, Glasgow Outcome Score, was collected after 6–12 months. We utilized a suspension bead antibody array analyzed on a FlexMap 3D Luminex platform to characterize 177 unique proteins in matched CSF and serum samples. In addition, we assessed BBB disruption using the CSF-serum albumin quotient (Q A), and performed Apolipoprotein E-genotyping as the latter has been linked to BBB function in the absence of trauma. We employed pathway-, cluster-, and proportional odds regression analyses. Key findings were validated in blood samples from an independent TBI cohort. Results TBI patients had an upregulation of structural CNS and neuroinflammatory pathways in both CSF and serum. In total, 114 proteins correlated with Q A, among which the top-correlated proteins were complement proteins. A cluster analysis revealed protein levels to be strongly associated with BBB integrity, but not carriage of the Apolipoprotein E4-variant. Among cluster-derived proteins, innate immune pathways were upregulated. Forty unique proteins emanated as novel independent predictors of clinical outcome, that individually explained ~ 10% additional model variance. Among proteins significantly different between TBI patients with intact or disrupted BBB, complement C9 in CSF (p = 0.014, ΔR 2 = 7.4%) and complement factor B in serum (p = 0.003, ΔR 2 = 9.2%) were independent outcome predictors also following step-down modelling. Conclusions This represents the largest concomitant CSF and serum proteomic profiling study so far reported in TBI, providing substantial support to the notion that neuroinflammatory markers, including complement activation, predicts BBB disruption and long-term outcome. Individual proteins identified here could potentially serve to refine current biomarker modelling or represent novel treatment targets in severe TBI. |
topic |
Traumatic brain injury Protein biomarkers Proteomics Neuroinflammation Blood–brain barrier Apolipoprotein E4 |
url |
https://doi.org/10.1186/s13054-021-03503-x |
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