Experimentally guided models reveal replication principles that shape the mutation distribution of RNA viruses
Life history theory posits that the sequence and timing of events in an organism's lifespan are fine-tuned by evolution to maximize the production of viable offspring. In a virus, a life history strategy is largely manifested in its replication mode. Here, we develop a stochastic mathematical m...
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doaj-3d990622421d4013bcf8d18935a041b42021-05-04T23:38:26ZengeLife Sciences Publications LtdeLife2050-084X2015-01-01410.7554/eLife.03753Experimentally guided models reveal replication principles that shape the mutation distribution of RNA virusesMichael B Schulte0Jeremy A Draghi1Joshua B Plotkin2Raul Andino3Tetrad Graduate Program, University of California, San Francisco, San Francisco, United States; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, United StatesDepartment of Biology, University of Pennsylvania, Philadelphia, United StatesDepartment of Biology, University of Pennsylvania, Philadelphia, United StatesDepartment of Microbiology and Immunology, University of California, San Francisco, San Francisco, United StatesLife history theory posits that the sequence and timing of events in an organism's lifespan are fine-tuned by evolution to maximize the production of viable offspring. In a virus, a life history strategy is largely manifested in its replication mode. Here, we develop a stochastic mathematical model to infer the replication mode shaping the structure and mutation distribution of a poliovirus population in an intact single infected cell. We measure production of RNA and poliovirus particles through the infection cycle, and use these data to infer the parameters of our model. We find that on average the viral progeny produced from each cell are approximately five generations removed from the infecting virus. Multiple generations within a single cell infection provide opportunities for significant accumulation of mutations per viral genome and for intracellular selection.https://elifesciences.org/articles/03753stochastic mathematical modelingpopulation structurevirus replicationRNA replication |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michael B Schulte Jeremy A Draghi Joshua B Plotkin Raul Andino |
spellingShingle |
Michael B Schulte Jeremy A Draghi Joshua B Plotkin Raul Andino Experimentally guided models reveal replication principles that shape the mutation distribution of RNA viruses eLife stochastic mathematical modeling population structure virus replication RNA replication |
author_facet |
Michael B Schulte Jeremy A Draghi Joshua B Plotkin Raul Andino |
author_sort |
Michael B Schulte |
title |
Experimentally guided models reveal replication principles that shape the mutation distribution of RNA viruses |
title_short |
Experimentally guided models reveal replication principles that shape the mutation distribution of RNA viruses |
title_full |
Experimentally guided models reveal replication principles that shape the mutation distribution of RNA viruses |
title_fullStr |
Experimentally guided models reveal replication principles that shape the mutation distribution of RNA viruses |
title_full_unstemmed |
Experimentally guided models reveal replication principles that shape the mutation distribution of RNA viruses |
title_sort |
experimentally guided models reveal replication principles that shape the mutation distribution of rna viruses |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2015-01-01 |
description |
Life history theory posits that the sequence and timing of events in an organism's lifespan are fine-tuned by evolution to maximize the production of viable offspring. In a virus, a life history strategy is largely manifested in its replication mode. Here, we develop a stochastic mathematical model to infer the replication mode shaping the structure and mutation distribution of a poliovirus population in an intact single infected cell. We measure production of RNA and poliovirus particles through the infection cycle, and use these data to infer the parameters of our model. We find that on average the viral progeny produced from each cell are approximately five generations removed from the infecting virus. Multiple generations within a single cell infection provide opportunities for significant accumulation of mutations per viral genome and for intracellular selection. |
topic |
stochastic mathematical modeling population structure virus replication RNA replication |
url |
https://elifesciences.org/articles/03753 |
work_keys_str_mv |
AT michaelbschulte experimentallyguidedmodelsrevealreplicationprinciplesthatshapethemutationdistributionofrnaviruses AT jeremyadraghi experimentallyguidedmodelsrevealreplicationprinciplesthatshapethemutationdistributionofrnaviruses AT joshuabplotkin experimentallyguidedmodelsrevealreplicationprinciplesthatshapethemutationdistributionofrnaviruses AT raulandino experimentallyguidedmodelsrevealreplicationprinciplesthatshapethemutationdistributionofrnaviruses |
_version_ |
1721476841664937984 |