Efficient Multiplex Genome Editing Induces Precise, and Self-Ligated Type Mutations in Tomato Plants

Several expression systems for multiple guide RNA (gRNAs) have been developed in the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) system to induce multiple-gene modifications in plants. Here, we evaluated mutation efficiencies in the tomato geno...

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Bibliographic Details
Main Authors: Ryosuke Hashimoto, Risa Ueta, Chihiro Abe, Yuriko Osakabe, Keishi Osakabe
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-07-01
Series:Frontiers in Plant Science
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2018.00916/full