Fast, non-toxic, and inexpensive n-butanol preparation of recombinant plasmids

Various commercial and non-commercial plasmid preparation protocols are currently available. However, the kits are expensive and many of the protocols contain toxic chemicals. Here we present a novel, optimized and, therefore, very advantageous plasmid preparation protocol using n-butanol. The prepa...

Full description

Bibliographic Details
Main Authors: Jürgen Brieger, Eberhard J. Weidt, Jochen Decker
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2000-06-01
Series:Genetics and Molecular Biology
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200010