Isolation of Endogenously Assembled RNA-Protein Complexes Using Affinity Purification Based on Streptavidin Aptamer S1

Isolation of Endogenously Assembled RNA-Protein Complexes Using Affinity Purification Based on Streptavidin Aptamer S1

Efficient isolation of endogenously assembled viral RNA-protein complexes is essential for understanding virus replication mechanisms. We have developed an affinity purification strategy based on an RNA affinity tag that allows large-scale preparation of native viral RNA-binding proteins (RBPs). The...

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Bibliographic Details
Main Authors: Yangchao Dong, Jing Yang, Wei Ye, Yuan Wang, Chuantao Ye, Daihui Weng, Huan Gao, Fanglin Zhang, Zhikai Xu, Yingfeng Lei
Format: Article
Language:English
Published: MDPI AG 2015-09-01
Series:International Journal of Molecular Sciences
Subjects:
living mammalian cells
DENV
streptavidin aptamer S1
affinity purification
RNA binding protein
40S ribosomal protein S8
Online Access:http://www.mdpi.com/1422-0067/16/9/22456

Internet

http://www.mdpi.com/1422-0067/16/9/22456

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