Differentiation of Bovine coronavirus (BCoV) genotypes by a restriction enzyme assay

This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus (BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was concluded that this assay can be used as a rapid tool for BCoV genotypes differentiati...

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Bibliographic Details
Main Authors: Sibele Pinheiro de Souza, Karen Miyuki Asano, Thaisa Lucas Sandri, Iracema Nunes de Barros, Leonardo José Richtzenhain, Paulo Eduardo Brandão
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia 2010-10-01
Series:Brazilian Journal of Microbiology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300034