Investigation of Biophysical Migration Parameters for Normal Tissue and Metastatic Cancer Cells After Radiotherapy Treatment
A large body of literature has demonstrated that the mechanical properties of microenvironment have a key role in regulating cancer cell adhesion, motility, and invasion. In this work, we have introduced two additional parameters, named cell trajectory extension and area traveled by cell, to describ...
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doaj-3e5e5a1cef2343b2b9500f3b603473ee2020-11-25T03:37:16ZengFrontiers Media S.A.Frontiers in Physics2296-424X2020-10-01810.3389/fphy.2020.575906575906Investigation of Biophysical Migration Parameters for Normal Tissue and Metastatic Cancer Cells After Radiotherapy TreatmentValeria Panzetta0Valeria Panzetta1Giuseppe La Verde2Giuseppe La Verde3Mariagabriella Pugliese4Mariagabriella Pugliese5Cecilia Arrichiello6Paolo Muto7Marco La Commara8Marco La Commara9Vittoria D'Avino10Paolo A. Netti11Paolo A. Netti12Sabato Fusco13Sabato Fusco14Centro di Ricerca Interdipartimentale sui Biomateriali, Università degli Studi di Napoli Federico II, Naples, ItalyCenter for Advanced Biomaterial for Health Care, Istituto Italiano di Tecnologia, Naples, ItalyIstituto Nazionale di Fisica Nucleare (INFN), Sezione di Naples, Naples, ItalyDipartimento di Farmacia, Università degli Studi di Napoli Federico II, Naples, ItalyIstituto Nazionale di Fisica Nucleare (INFN), Sezione di Naples, Naples, ItalyDipartimento di Fisica “Ettore Pancini”, Università degli Studi di Napoli Federico II, Naples, ItalyRadiotherapy Unit, Istituto Nazionale Tumori-Istituti di Ricovero e Cura a Carattere Scientifico (IRCCS)-Fondazione “G. Pascale”, Naples, ItalyRadiotherapy Unit, Istituto Nazionale Tumori-Istituti di Ricovero e Cura a Carattere Scientifico (IRCCS)-Fondazione “G. Pascale”, Naples, ItalyIstituto Nazionale di Fisica Nucleare (INFN), Sezione di Naples, Naples, ItalyDipartimento di Farmacia, Università degli Studi di Napoli Federico II, Naples, ItalyIstituto Nazionale di Fisica Nucleare (INFN), Sezione di Naples, Naples, ItalyCentro di Ricerca Interdipartimentale sui Biomateriali, Università degli Studi di Napoli Federico II, Naples, ItalyCenter for Advanced Biomaterial for Health Care, Istituto Italiano di Tecnologia, Naples, ItalyCentro di Ricerca Interdipartimentale sui Biomateriali, Università degli Studi di Napoli Federico II, Naples, ItalyCenter for Advanced Biomaterial for Health Care, Istituto Italiano di Tecnologia, Naples, ItalyA large body of literature has demonstrated that the mechanical properties of microenvironment have a key role in regulating cancer cell adhesion, motility, and invasion. In this work, we have introduced two additional parameters, named cell trajectory extension and area traveled by cell, to describe the tendency of normal tissue and metastatic cancer cells to move in a directional way when they interact with physio-pathological substrates, characterized by stiffnesses of 1–13 kPa, before and after treatment with 2 doses of X-rays (2 and 10 Gy). We interpreted these data by evaluating also the impact of substrate stiffness on 2 morphological parameters which indicate not only the state of cell adhesion, but also cell polarization, prerequisite to directional movement, and the formation of protrusions over cell perimeters. We believe that a so wide analysis can give an efficient and easily readable overview of effects of radiation therapy on cell-ECM crosstalk when used as therapeutic agent.https://www.frontiersin.org/article/10.3389/fphy.2020.575906/fullbreast cancermechanobiologycell motility analysisextracellular matrix stiffnessradiotherapy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Valeria Panzetta Valeria Panzetta Giuseppe La Verde Giuseppe La Verde Mariagabriella Pugliese Mariagabriella Pugliese Cecilia Arrichiello Paolo Muto Marco La Commara Marco La Commara Vittoria D'Avino Paolo A. Netti Paolo A. Netti Sabato Fusco Sabato Fusco |
spellingShingle |
Valeria Panzetta Valeria Panzetta Giuseppe La Verde Giuseppe La Verde Mariagabriella Pugliese Mariagabriella Pugliese Cecilia Arrichiello Paolo Muto Marco La Commara Marco La Commara Vittoria D'Avino Paolo A. Netti Paolo A. Netti Sabato Fusco Sabato Fusco Investigation of Biophysical Migration Parameters for Normal Tissue and Metastatic Cancer Cells After Radiotherapy Treatment Frontiers in Physics breast cancer mechanobiology cell motility analysis extracellular matrix stiffness radiotherapy |
author_facet |
Valeria Panzetta Valeria Panzetta Giuseppe La Verde Giuseppe La Verde Mariagabriella Pugliese Mariagabriella Pugliese Cecilia Arrichiello Paolo Muto Marco La Commara Marco La Commara Vittoria D'Avino Paolo A. Netti Paolo A. Netti Sabato Fusco Sabato Fusco |
author_sort |
Valeria Panzetta |
title |
Investigation of Biophysical Migration Parameters for Normal Tissue and Metastatic Cancer Cells After Radiotherapy Treatment |
title_short |
Investigation of Biophysical Migration Parameters for Normal Tissue and Metastatic Cancer Cells After Radiotherapy Treatment |
title_full |
Investigation of Biophysical Migration Parameters for Normal Tissue and Metastatic Cancer Cells After Radiotherapy Treatment |
title_fullStr |
Investigation of Biophysical Migration Parameters for Normal Tissue and Metastatic Cancer Cells After Radiotherapy Treatment |
title_full_unstemmed |
Investigation of Biophysical Migration Parameters for Normal Tissue and Metastatic Cancer Cells After Radiotherapy Treatment |
title_sort |
investigation of biophysical migration parameters for normal tissue and metastatic cancer cells after radiotherapy treatment |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physics |
issn |
2296-424X |
publishDate |
2020-10-01 |
description |
A large body of literature has demonstrated that the mechanical properties of microenvironment have a key role in regulating cancer cell adhesion, motility, and invasion. In this work, we have introduced two additional parameters, named cell trajectory extension and area traveled by cell, to describe the tendency of normal tissue and metastatic cancer cells to move in a directional way when they interact with physio-pathological substrates, characterized by stiffnesses of 1–13 kPa, before and after treatment with 2 doses of X-rays (2 and 10 Gy). We interpreted these data by evaluating also the impact of substrate stiffness on 2 morphological parameters which indicate not only the state of cell adhesion, but also cell polarization, prerequisite to directional movement, and the formation of protrusions over cell perimeters. We believe that a so wide analysis can give an efficient and easily readable overview of effects of radiation therapy on cell-ECM crosstalk when used as therapeutic agent. |
topic |
breast cancer mechanobiology cell motility analysis extracellular matrix stiffness radiotherapy |
url |
https://www.frontiersin.org/article/10.3389/fphy.2020.575906/full |
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