SYNONYMOUS CONDON USAGE BIAS AND OVEREXPRESSION OF A SYNTHETIC xynB GENE FROM Aspergillus niger NL-1 IN Pichia pastoris

SYNONYMOUS CONDON USAGE BIAS AND OVEREXPRESSION OF A SYNTHETIC xynB GENE FROM Aspergillus niger NL-1 IN Pichia pastoris

To further improve the expression level of recombinant xylanase in Pichia pastoris, the xynB gene, encoding the mature peptide from Aspergillus niger NL-1, was designed and synthesized based on the synonymous condon bias of P. pastoris and optimized G+C content. 155 nucleotides were changed, and the...

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Bibliographic Details
Main Authors: Fei Li, Shiyi Yang, Linguo Zhao, Qi Li, Jianjun Pei
Format: Article
Language:English
Published: North Carolina State University 2012-02-01
Series:BioResources
Subjects:
Endoxylanase xynB
Synonymous condon
Synthesis
Over expression
Aspergillus niger
Online Access:http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_07_2_2330_Li_YZLP_Synonymous_condon_Usage_Bias_Overexpression/1510

Internet

http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_07_2_2330_Li_YZLP_Synonymous_condon_Usage_Bias_Overexpression/1510

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