Impact of Preservation Method and 16S rRNA Hypervariable Region on Gut Microbiota Profiling

Impact of Preservation Method and 16S rRNA Hypervariable Region on Gut Microbiota Profiling

Large-scale human microbiota studies require specimens collected from multiple sites and/or time points to maximize detection of the small effects in microbe-host interactions. However, batch biases caused by experimental protocols, such as sample collection, massively parallel sequencing, and bioin...

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Bibliographic Details
Main Authors: Zigui Chen, Pak Chun Hui, Mamie Hui, Yun Kit Yeoh, Po Yee Wong, Martin C. W. Chan, Martin C. S. Wong, Siew C. Ng, Francis K. L. Chan, Paul K. S. Chan
Format: Article
Language:English
Published: American Society for Microbiology 2019-02-01
Series:mSystems
Subjects:
16S rRNA gene
amplicon sequencing
bacterial culture
gut microbiota
preservative
Online Access:https://doi.org/10.1128/mSystems.00271-18

Internet

https://doi.org/10.1128/mSystems.00271-18

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