Preparation and culture of bone marrow-derived macrophages from mice for functional analysis

Summary: The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures....

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Bibliographic Details
Main Authors: Gotaro Toda, Toshimasa Yamauchi, Takashi Kadowaki, Kohjiro Ueki
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302331