Preparation and culture of bone marrow-derived macrophages from mice for functional analysis
Summary: The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures....
Main Authors: | Gotaro Toda, Toshimasa Yamauchi, Takashi Kadowaki, Kohjiro Ueki |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2021-03-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720302331 |
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