How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments

How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments

We have examined the imprecision in the estimation of PCR efficiency by means of standard curves based on strategic experimental design with large number of technical replicates. In particular, how robust this estimation is in terms of a commonly varying factors: the instrument used, the number of t...

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Bibliographic Details
Main Authors: David Svec, Ales Tichopad, Vendula Novosadova, Michael W. Pfaffl, Mikael Kubista
Format: Article
Language:English
Published: Elsevier 2015-03-01
Series:Biomolecular Detection and Quantification
Subjects:
Real-time quantitative PCR
qPCR
Amplification efficiency
Standard curve
Dilution series
qPCR assay validation
Online Access:http://www.sciencedirect.com/science/article/pii/S2214753515000169

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http://www.sciencedirect.com/science/article/pii/S2214753515000169

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