Assessment of Intracellular Insulin Content during All Steps of Human Islet Isolation Procedure
This study investigated the recovery of pancreatic insulin content during human islet isolation prior to and after digestion-filtration, continuous Hanks-Ficoll gradient purification ( n = 20), and 3–4 day culture at 22°C ( n = 6). The native insulin content varied in a wide range from 28.4 U to 360...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
SAGE Publishing
1998-09-01
|
Series: | Cell Transplantation |
Online Access: | https://doi.org/10.1177/096368979800700508 |