Assessment of Intracellular Insulin Content during All Steps of Human Islet Isolation Procedure

This study investigated the recovery of pancreatic insulin content during human islet isolation prior to and after digestion-filtration, continuous Hanks-Ficoll gradient purification ( n = 20), and 3–4 day culture at 22°C ( n = 6). The native insulin content varied in a wide range from 28.4 U to 360...

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Bibliographic Details
Main Authors: Heide Brandhorst, Daniel Brandhorst, Mathias D. Brendel, Bernhard J. Hering, Reinhard G. Bretzel
Format: Article
Language:English
Published: SAGE Publishing 1998-09-01
Series:Cell Transplantation
Online Access:https://doi.org/10.1177/096368979800700508