Ultrasensitive Quantification of Cytokine Proteins in Single Lymphocytes From Human Blood Following ex-vivo Stimulation

Ultrasensitive Quantification of Cytokine Proteins in Single Lymphocytes From Human Blood Following ex-vivo Stimulation

In this study we demonstrate the feasibility of direct, quantitative measurement of cytokine proteins in single human CD8 lymphocytes from fresh peripheral blood of healthy donors following a brief ex vivo stimulation. Cytokine-secreting cells were identified using cell surface “catch” reagents and...

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Bibliographic Details
Main Authors: Ankit Saxena, Pradeep K. Dagur, Alisha Desai, John Philip McCoy
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-10-01
Series:Frontiers in Immunology
Subjects:
IFN-γ
TNF-α
T cell
SiMoA
flow cytometry
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2018.02462/full

Internet

https://www.frontiersin.org/article/10.3389/fimmu.2018.02462/full

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