Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture
Improved culture methods are needed to reliably grow human pluripotent stem cells (hPSCs) on a large scale. Here, the authors identify a xeno-free medium with a supplement of Inter-α-inhibitor that supports long-term propagation and improved single-cell passaging of hPSCs on uncoated plastic.
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2016-07-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/ncomms12170 |