Integrated Metabolomics and Proteomics Analysis Revealed Second Messenger System Disturbance in Hippocampus of Chronic Social Defeat Stress Rat
Depression is a common and disabling mental disorder characterized by high disability and mortality, but its physiopathology remains unclear. In this study, we combined a non-targeted gas chromatography-mass spectrometry (GC-MS)-based metabolomic approach and isobaric tags for relative and absolute...
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doaj-4048491d38a64c8f8be1a9b592f6e0ad2020-11-24T21:21:35ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2019-03-011310.3389/fnins.2019.00247437510Integrated Metabolomics and Proteomics Analysis Revealed Second Messenger System Disturbance in Hippocampus of Chronic Social Defeat Stress RatLi-Ning Yang0Li-Ning Yang1Jun-Cai Pu2Jun-Cai Pu3Lan-Xiang Liu4Lan-Xiang Liu5Guo-Wei Wang6Xin-Yu Zhou7Yu-Qing Zhang8Yi-Yun Liu9Yi-Yun Liu10Peng Xie11Peng Xie12Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaInstitute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing, ChinaDepartment of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaInstitute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing, ChinaDepartment of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaInstitute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing, ChinaSchool of Clinical Medicine, Ningxia Medical University, Yinchuan, ChinaDepartment of Psychiatry, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Neurology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaInstitute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing, ChinaDepartment of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaInstitute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing, ChinaDepression is a common and disabling mental disorder characterized by high disability and mortality, but its physiopathology remains unclear. In this study, we combined a non-targeted gas chromatography-mass spectrometry (GC-MS)-based metabolomic approach and isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis to elucidate metabolite and protein alterations in the hippocampus of rat after chronic social defeat stress (CSDS), an extensively used animal model of depression. Ingenuity pathway analysis (IPA) was conducted to integrate underlying relationships among differentially expressed metabolites and proteins. Twenty-five significantly different expressed metabolites and 234 differentially expressed proteins were identified between CSDS and control groups. IPA canonical pathways and network analyses revealed that intracellular second messenger/signal transduction cascades were most significantly altered in the hippocampus of CSDS rats, including cyclic adenosine monophosphate (cAMP), phosphoinositol, tyrosine kinase, and arachidonic acid systems. These results provide a better understanding of biological mechanisms underlying depression, and may help identify potential targets for novel antidepressants.https://www.frontiersin.org/article/10.3389/fnins.2019.00247/fulldepressionsocial defeatproteomicsmetabolomicssecond-messenger |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li-Ning Yang Li-Ning Yang Jun-Cai Pu Jun-Cai Pu Lan-Xiang Liu Lan-Xiang Liu Guo-Wei Wang Xin-Yu Zhou Yu-Qing Zhang Yi-Yun Liu Yi-Yun Liu Peng Xie Peng Xie |
spellingShingle |
Li-Ning Yang Li-Ning Yang Jun-Cai Pu Jun-Cai Pu Lan-Xiang Liu Lan-Xiang Liu Guo-Wei Wang Xin-Yu Zhou Yu-Qing Zhang Yi-Yun Liu Yi-Yun Liu Peng Xie Peng Xie Integrated Metabolomics and Proteomics Analysis Revealed Second Messenger System Disturbance in Hippocampus of Chronic Social Defeat Stress Rat Frontiers in Neuroscience depression social defeat proteomics metabolomics second-messenger |
author_facet |
Li-Ning Yang Li-Ning Yang Jun-Cai Pu Jun-Cai Pu Lan-Xiang Liu Lan-Xiang Liu Guo-Wei Wang Xin-Yu Zhou Yu-Qing Zhang Yi-Yun Liu Yi-Yun Liu Peng Xie Peng Xie |
author_sort |
Li-Ning Yang |
title |
Integrated Metabolomics and Proteomics Analysis Revealed Second Messenger System Disturbance in Hippocampus of Chronic Social Defeat Stress Rat |
title_short |
Integrated Metabolomics and Proteomics Analysis Revealed Second Messenger System Disturbance in Hippocampus of Chronic Social Defeat Stress Rat |
title_full |
Integrated Metabolomics and Proteomics Analysis Revealed Second Messenger System Disturbance in Hippocampus of Chronic Social Defeat Stress Rat |
title_fullStr |
Integrated Metabolomics and Proteomics Analysis Revealed Second Messenger System Disturbance in Hippocampus of Chronic Social Defeat Stress Rat |
title_full_unstemmed |
Integrated Metabolomics and Proteomics Analysis Revealed Second Messenger System Disturbance in Hippocampus of Chronic Social Defeat Stress Rat |
title_sort |
integrated metabolomics and proteomics analysis revealed second messenger system disturbance in hippocampus of chronic social defeat stress rat |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neuroscience |
issn |
1662-453X |
publishDate |
2019-03-01 |
description |
Depression is a common and disabling mental disorder characterized by high disability and mortality, but its physiopathology remains unclear. In this study, we combined a non-targeted gas chromatography-mass spectrometry (GC-MS)-based metabolomic approach and isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis to elucidate metabolite and protein alterations in the hippocampus of rat after chronic social defeat stress (CSDS), an extensively used animal model of depression. Ingenuity pathway analysis (IPA) was conducted to integrate underlying relationships among differentially expressed metabolites and proteins. Twenty-five significantly different expressed metabolites and 234 differentially expressed proteins were identified between CSDS and control groups. IPA canonical pathways and network analyses revealed that intracellular second messenger/signal transduction cascades were most significantly altered in the hippocampus of CSDS rats, including cyclic adenosine monophosphate (cAMP), phosphoinositol, tyrosine kinase, and arachidonic acid systems. These results provide a better understanding of biological mechanisms underlying depression, and may help identify potential targets for novel antidepressants. |
topic |
depression social defeat proteomics metabolomics second-messenger |
url |
https://www.frontiersin.org/article/10.3389/fnins.2019.00247/full |
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