Development of a Broad-Range 23S rDNA Real-Time PCR Assay for the Detection and Quantification of Pathogenic Bacteria in Human Whole Blood and Plasma Specimens

Molecular methods are important tools in the diagnosis of bloodstream bacterial infections, in particular in patients treated with antimicrobial therapy, due to their quick turn-around time. Here we describe a new broad-range real-time PCR targeting the 23S rDNA gene and capable to detect as low as...

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Bibliographic Details
Main Authors: Paolo Gaibani, Mara Mariconti, Gloria Bua, Sonia Bonora, Davide Sassera, Maria Paola Landini, Patrizia Mulatto, Stefano Novati, Claudio Bandi, Vittorio Sambri
Format: Article
Language:English
Published: Hindawi Limited 2013-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2013/264651