Microscale thermophoresis as a powerful tool for screening glycosyltransferases involved in cell wall biosynthesis

Abstract Background Identification and characterization of key enzymes associated with cell wall biosynthesis and modification is fundamental to gain insights into cell wall dynamics. However, it is a challenge that activity assays of glycosyltransferases are very low throughput and acceptor substra...

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Bibliographic Details
Main Authors: Wanchen Shao, Rita Sharma, Mads H. Clausen, Henrik V. Scheller
Format: Article
Language:English
Published: BMC 2020-07-01
Series:Plant Methods
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13007-020-00641-1