Addressing confounding artifacts in reconstruction of gene co-expression networks
Abstract Gene co-expression networks capture biological relationships between genes and are important tools in predicting gene function and understanding disease mechanisms. We show that technical and biological artifacts in gene expression data confound commonly used network reconstruction algorith...
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Online Access: | http://link.springer.com/article/10.1186/s13059-019-1700-9 |
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doaj-40fe5e619182489ba2397e77666f3ae32020-11-25T03:27:20ZengBMCGenome Biology1474-760X2019-05-012011610.1186/s13059-019-1700-9Addressing confounding artifacts in reconstruction of gene co-expression networksPrincy Parsana0Claire Ruberman1Andrew E. Jaffe2Michael C. Schatz3Alexis Battle4Jeffrey T. Leek5Department of Computer Science, Johns Hopkins UniversityDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public HealthDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public HealthDepartment of Computer Science, Johns Hopkins UniversityDepartment of Computer Science, Johns Hopkins UniversityDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public HealthAbstract Gene co-expression networks capture biological relationships between genes and are important tools in predicting gene function and understanding disease mechanisms. We show that technical and biological artifacts in gene expression data confound commonly used network reconstruction algorithms. We demonstrate theoretically, in simulation, and empirically, that principal component correction of gene expression measurements prior to network inference can reduce false discoveries. Using data from the GTEx project in multiple tissues, we show that this approach reduces false discoveries beyond correcting only for known confounders.http://link.springer.com/article/10.1186/s13059-019-1700-9 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Princy Parsana Claire Ruberman Andrew E. Jaffe Michael C. Schatz Alexis Battle Jeffrey T. Leek |
spellingShingle |
Princy Parsana Claire Ruberman Andrew E. Jaffe Michael C. Schatz Alexis Battle Jeffrey T. Leek Addressing confounding artifacts in reconstruction of gene co-expression networks Genome Biology |
author_facet |
Princy Parsana Claire Ruberman Andrew E. Jaffe Michael C. Schatz Alexis Battle Jeffrey T. Leek |
author_sort |
Princy Parsana |
title |
Addressing confounding artifacts in reconstruction of gene co-expression networks |
title_short |
Addressing confounding artifacts in reconstruction of gene co-expression networks |
title_full |
Addressing confounding artifacts in reconstruction of gene co-expression networks |
title_fullStr |
Addressing confounding artifacts in reconstruction of gene co-expression networks |
title_full_unstemmed |
Addressing confounding artifacts in reconstruction of gene co-expression networks |
title_sort |
addressing confounding artifacts in reconstruction of gene co-expression networks |
publisher |
BMC |
series |
Genome Biology |
issn |
1474-760X |
publishDate |
2019-05-01 |
description |
Abstract Gene co-expression networks capture biological relationships between genes and are important tools in predicting gene function and understanding disease mechanisms. We show that technical and biological artifacts in gene expression data confound commonly used network reconstruction algorithms. We demonstrate theoretically, in simulation, and empirically, that principal component correction of gene expression measurements prior to network inference can reduce false discoveries. Using data from the GTEx project in multiple tissues, we show that this approach reduces false discoveries beyond correcting only for known confounders. |
url |
http://link.springer.com/article/10.1186/s13059-019-1700-9 |
work_keys_str_mv |
AT princyparsana addressingconfoundingartifactsinreconstructionofgenecoexpressionnetworks AT claireruberman addressingconfoundingartifactsinreconstructionofgenecoexpressionnetworks AT andrewejaffe addressingconfoundingartifactsinreconstructionofgenecoexpressionnetworks AT michaelcschatz addressingconfoundingartifactsinreconstructionofgenecoexpressionnetworks AT alexisbattle addressingconfoundingartifactsinreconstructionofgenecoexpressionnetworks AT jeffreytleek addressingconfoundingartifactsinreconstructionofgenecoexpressionnetworks |
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