Biosynthesis and Immobilization of Biofunctional Allophycocyanin

The holo-allophycocyanin-α subunit, which has various reported pharmacological uses, was biosynthesized with both Strep-II-tag and His-tag at the N-terminal in Escherichia coli. The streptavidin-binding ability resulting from the Strep II-tag was confirmed by Western blot. Additionally, the metal-ch...

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Bibliographic Details
Main Authors: Yingjie Chen, Shaofang Liu, Yulin Cui, Peng Jiang, Huaxin Chen, Fuchao Li, Song Qin
Format: Article
Language:English
Published: Hindawi Limited 2011-01-01
Series:Evidence-Based Complementary and Alternative Medicine
Online Access:http://dx.doi.org/10.1155/2011/751452