Biosynthesis and Immobilization of Biofunctional Allophycocyanin
The holo-allophycocyanin-α subunit, which has various reported pharmacological uses, was biosynthesized with both Strep-II-tag and His-tag at the N-terminal in Escherichia coli. The streptavidin-binding ability resulting from the Strep II-tag was confirmed by Western blot. Additionally, the metal-ch...
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doaj-4227f5cab2a24bbf8d5f2aaf576a4c8c2020-11-24T21:47:45ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882011-01-01201110.1155/2011/751452751452Biosynthesis and Immobilization of Biofunctional AllophycocyaninYingjie Chen0Shaofang Liu1Yulin Cui2Peng Jiang3Huaxin Chen4Fuchao Li5Song Qin6Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaThe holo-allophycocyanin-α subunit, which has various reported pharmacological uses, was biosynthesized with both Strep-II-tag and His-tag at the N-terminal in Escherichia coli. The streptavidin-binding ability resulting from the Strep II-tag was confirmed by Western blot. Additionally, the metal-chelating ability deriving from the His-tag not only facilitated its purification by immobilized metal-ion affinity chromatography but also promoted its immobilization on Zn (II)-decorated silica-coated magnetic nanoparticles. The holo-allophycocyanin-α subunit with streptavidin-binding ability was thereby immobilized on magnetic nanoparticles. Magnetic nanoparticles are promising as drug delivery vehicles for targeting and locating at tumors. Thus, based on genetic engineering and nanotechnology, we provide a potential strategy to facilitate the biomodification and targeted delivery of pharmacological proteins.http://dx.doi.org/10.1155/2011/751452 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yingjie Chen Shaofang Liu Yulin Cui Peng Jiang Huaxin Chen Fuchao Li Song Qin |
spellingShingle |
Yingjie Chen Shaofang Liu Yulin Cui Peng Jiang Huaxin Chen Fuchao Li Song Qin Biosynthesis and Immobilization of Biofunctional Allophycocyanin Evidence-Based Complementary and Alternative Medicine |
author_facet |
Yingjie Chen Shaofang Liu Yulin Cui Peng Jiang Huaxin Chen Fuchao Li Song Qin |
author_sort |
Yingjie Chen |
title |
Biosynthesis and Immobilization of Biofunctional Allophycocyanin |
title_short |
Biosynthesis and Immobilization of Biofunctional Allophycocyanin |
title_full |
Biosynthesis and Immobilization of Biofunctional Allophycocyanin |
title_fullStr |
Biosynthesis and Immobilization of Biofunctional Allophycocyanin |
title_full_unstemmed |
Biosynthesis and Immobilization of Biofunctional Allophycocyanin |
title_sort |
biosynthesis and immobilization of biofunctional allophycocyanin |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2011-01-01 |
description |
The holo-allophycocyanin-α subunit, which has various reported pharmacological uses, was biosynthesized with both Strep-II-tag and His-tag at the N-terminal in Escherichia coli. The streptavidin-binding ability resulting from the Strep II-tag was confirmed by Western blot. Additionally, the metal-chelating ability deriving from the His-tag not only facilitated its purification by immobilized metal-ion affinity chromatography but also promoted its immobilization on Zn (II)-decorated silica-coated magnetic nanoparticles. The holo-allophycocyanin-α subunit with streptavidin-binding ability was thereby immobilized on magnetic nanoparticles. Magnetic nanoparticles are promising as drug delivery vehicles for targeting and locating at tumors. Thus, based on genetic engineering and nanotechnology, we provide a potential strategy to facilitate the biomodification and targeted delivery of pharmacological proteins. |
url |
http://dx.doi.org/10.1155/2011/751452 |
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