Biosynthesis and Immobilization of Biofunctional Allophycocyanin
The holo-allophycocyanin-α subunit, which has various reported pharmacological uses, was biosynthesized with both Strep-II-tag and His-tag at the N-terminal in Escherichia coli. The streptavidin-binding ability resulting from the Strep II-tag was confirmed by Western blot. Additionally, the metal-ch...
Main Authors: | Yingjie Chen, Shaofang Liu, Yulin Cui, Peng Jiang, Huaxin Chen, Fuchao Li, Song Qin |
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Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2011-01-01
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Series: | Evidence-Based Complementary and Alternative Medicine |
Online Access: | http://dx.doi.org/10.1155/2011/751452 |
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