Increasing plasmid transformation efficiency of natural spizizen method in Bacillus Subtilis by a cell permeable peptide

Introduction: Some of bacterial species are able to uptake DNA molecule from environment, the yield of this process depends on some conditions such as plasmid size and host type. In the case of Bacillus subtilis, DNA uptake has low efficacy. Using Spizizen minimal medium is common method in plasmid...

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Bibliographic Details
Main Authors: Mehrdad Moosazadeh Moghaddam, Mohammad Eftekhari, Mohammad Heiat, Hossein Aghamollaei
Format: Article
Language:English
Published: University of Isfahan 2013-01-01
Series:Biological Journal of Microorganism
Subjects:
Online Access:http://uijs.ui.ac.ir/bjm/browse.php?a_id=101&slc_lang=fa&sid=1&ftxt=1