Improved in Vitro Folding of the Y2 G Protein-Coupled Receptor into Bicelles
Prerequisite for structural studies on G protein-coupled receptors is the preparation of highly concentrated, stable, and biologically active receptor samples in milligram amounts of protein. Here, we present an improved protocol for Escherichia coli expression, functional refolding, and reconstitut...
Main Authors: | , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2018-01-01
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Series: | Frontiers in Molecular Biosciences |
Subjects: | |
Online Access: | http://journal.frontiersin.org/article/10.3389/fmolb.2017.00100/full |