Improved in Vitro Folding of the Y2 G Protein-Coupled Receptor into Bicelles

Improved in Vitro Folding of the Y2 G Protein-Coupled Receptor into Bicelles

Prerequisite for structural studies on G protein-coupled receptors is the preparation of highly concentrated, stable, and biologically active receptor samples in milligram amounts of protein. Here, we present an improved protocol for Escherichia coli expression, functional refolding, and reconstitut...

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Bibliographic Details
Main Authors: Peter Schmidt, Brian J. Bender, Anette Kaiser, Khushboo Gulati, Holger A. Scheidt, Heidi E. Hamm, Jens Meiler, Annette G. Beck-Sickinger, Daniel Huster
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-01-01
Series:Frontiers in Molecular Biosciences
Subjects:
GPCR
NPY
bicelles
folding
NMR
Online Access:http://journal.frontiersin.org/article/10.3389/fmolb.2017.00100/full

Internet

http://journal.frontiersin.org/article/10.3389/fmolb.2017.00100/full

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