Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system
The use of base editing enables precise genetic modifications in model animals. Here the authors show high efficient single-base editing in zebrafish using modified Cas9 and its VQR variant with an altered PAM specificity.
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2017-07-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-017-00175-6 |