i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases

i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases

Abstract We present a robust method called improved-Genome editing via Oviductal Nucleic Acids Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0.7 embryos via in situ electroporation. The method generates mouse models containing single-base changes, kilobase-sized deletions, and knock...

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Bibliographic Details
Main Authors: Masato Ohtsuka, Masahiro Sato, Hiromi Miura, Shuji Takabayashi, Makoto Matsuyama, Takayuki Koyano, Naomi Arifin, Shingo Nakamura, Kenta Wada, Channabasavaiah B. Gurumurthy
Format: Article
Language:English
Published: BMC 2018-02-01
Series:Genome Biology
Subjects:
In vivo electroporation
CRISPR
GONAD
Knock-in
Transgenic mouse
Long ssDNA
Online Access:http://link.springer.com/article/10.1186/s13059-018-1400-x

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http://link.springer.com/article/10.1186/s13059-018-1400-x

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