Precise single base substitution in the shibire gene by CRISPR/Cas9-mediated homology directed repair in Bactrocera tryoni

Abstract Background Pest eradication using the Sterile Insect Technique (SIT) involves high-density releases of sterilized males that mate with wild females and ultimately suppress the population. Sterilized females are not required for SIT and their removal or separation from males prior to release...

Full description

Bibliographic Details
Main Authors: Amanda Choo, Elisabeth Fung, Isabel Y. Chen, Robert Saint, Peter Crisp, Simon W. Baxter
Format: Article
Language:English
Published: BMC 2020-12-01
Series:BMC Genetics
Subjects:
Online Access:https://doi.org/10.1186/s12863-020-00934-3