Systematic comparison of small RNA library preparation protocols for next-generation sequencing

Systematic comparison of small RNA library preparation protocols for next-generation sequencing

Abstract Background Next-generation sequencing technologies have revolutionized the study of small RNAs (sRNAs) on a genome-wide scale. However, classical sRNA library preparation methods introduce serious bias, mainly during adapter ligation steps. Several types of sRNA including plant microRNAs (m...

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Bibliographic Details
Main Authors: Cloelia Dard-Dascot, Delphine Naquin, Yves d’Aubenton-Carafa, Karine Alix, Claude Thermes, Erwin van Dijk
Format: Article
Language:English
Published: BMC 2018-02-01
Series:BMC Genomics
Subjects:
Small RNA
Bias
Library preparation
Next-generation sequencing
NGS
2’-O-methyl RNA
Online Access:http://link.springer.com/article/10.1186/s12864-018-4491-6

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http://link.springer.com/article/10.1186/s12864-018-4491-6

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