A Sensitive Whole Blood Assay Detects Antigen-Stimulated Cytokine Release From CD4+ T Cells and Facilitates Immunomonitoring in a Phase 2 Clinical Trial of Nexvax2 in Coeliac Disease

A Sensitive Whole Blood Assay Detects Antigen-Stimulated Cytokine Release From CD4+ T Cells and Facilitates Immunomonitoring in a Phase 2 Clinical Trial of Nexvax2 in Coeliac Disease

Improved blood tests assessing the functional status of rare gluten-specific CD4+ T cells are needed to effectively monitor experimental therapies for coeliac disease (CD). Our aim was to develop a simple, but highly sensitive cytokine release assay (CRA) for gluten-specific CD4+ T cells that did no...

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Bibliographic Details
Main Authors: Melinda Y. Hardy, Gautam Goel, Amy K. Russell, Swee Lin G. Chen Yi Mei, Gregor J. E. Brown, Suyue Wang, Evan Szymczak, Ruan Zhang, Kaela E. Goldstein, Kristin M. Neff, Leslie J. Williams, Kenneth E. Truitt, John L. Dzuris, Jason A. Tye-Din, Robert P. Anderson
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-05-01
Series:Frontiers in Immunology
Subjects:
coeliac disease
T cells
cytokines
cytokine release assay
IL-2
diagnosis
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2021.661622/full

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https://www.frontiersin.org/articles/10.3389/fimmu.2021.661622/full

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