Highly sensitive assay of HMG-CoA reductase activity by LC-ESI-MS/MS

Highly sensitive assay of HMG-CoA reductase activity by LC-ESI-MS/MS

We have developed a new sensitive and specific nonradioisotope assay method to measure the activity of HMG-CoA reductase, the rate-controlling enzyme in the cholesterol biosynthetic pathway. This method was based upon a stable isotope dilution technique by liquid chromatography-tandem mass spectrome...

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Bibliographic Details
Main Authors: Honda Akira, Mizokami Yuji, Matsuzaki Yasushi, Ikegami Tadashi, Doy Mikio, Miyazaki Hiroshi
Format: Article
Language:English
Published: Elsevier 2007-05-01
Series:Journal of Lipid Research
Subjects:
cholesterol biosynthesis
mevalonic acid
mevalonolactone
liquid chromatography-electrospray ionization-tandem mass spectrometry
3-hydroxy-3-methylglutaryl-coenzyme A reductase
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520435679

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http://www.sciencedirect.com/science/article/pii/S0022227520435679

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