Chondrotoxicity of Local Anesthetics: Liposomal Bupivacaine Is Less Chondrotoxic than Standard Bupivacaine
Objective. *e purpose of this study is to determine whether (1) liposomal bupivacaine is chondrotoxic; (2) the chondrotoxicity of liposomal bupivacaine differs from standard bupivacaine; and (3) chondrotoxic effects are time dependent. Materials and Methods. We obtained 72 10 mm articular cartilag...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2020-01-01
|
Series: | Advances in Pharmacological and Pharmaceutical Sciences |
Online Access: | https://www.hindawi.com/journals/aps/2020/5794187/ |
id |
doaj-45395c1687594e98a6fde6b0e8e36e2f |
---|---|
record_format |
Article |
spelling |
doaj-45395c1687594e98a6fde6b0e8e36e2f2020-11-25T03:15:07ZengHindawi LimitedAdvances in Pharmacological and Pharmaceutical Sciences2633-46902020-01-01202010.1155/2020/5794187Chondrotoxicity of Local Anesthetics: Liposomal Bupivacaine Is Less Chondrotoxic than Standard BupivacaineTravis Farmer0S. Craig Morris1https://orcid.org/0000-0003-1034-6219Robert Quigley2Nirav H. Amin3Montri D. Wongworawat4Hasan M. Syed5Department of Orthopaedic Surgery, Loma Linda University, 11406 Loma Linda Drive, Suite 218, Loma Linda, CA 92354, USADepartment of Orthopaedic Surgery, Loma Linda University, 11406 Loma Linda Drive, Suite 218, Loma Linda, CA 92354, USADepartment of Pediatric Orthopaedic Surgery, Children’s Hospital at Erlanger, 910 Blackford Street, Chattanooga, TN 37403, USADepartment of Orthopaedic Surgery, VA Loma Linda Healthcare System, 11201 Benton Street, Loma Linda, CA 92357, USADepartment of Orthopaedic Surgery, Loma Linda University, 11406 Loma Linda Drive, Suite 218, Loma Linda, CA 92354, USADepartment of Orthopaedic Surgery, Loma Linda University, 11406 Loma Linda Drive, Suite 218, Loma Linda, CA 92354, USAObjective. *e purpose of this study is to determine whether (1) liposomal bupivacaine is chondrotoxic; (2) the chondrotoxicity of liposomal bupivacaine differs from standard bupivacaine; and (3) chondrotoxic effects are time dependent. Materials and Methods. We obtained 72 10 mm articular cartilage plugs from 12 fresh bovine distal femoral knee joints and exposed them to either saline, 0.5% bupivacaine, or liposomal bupivacaine for either 30 or 90 minutes. Twenty-four hours after treatment, chondrocyte viability was measured with the use of a fluorescent live/dead assay. An ANOVA test of variance was performed followed by a Holm–Sidak test to make pairwise comparisons across conditions. Student’s t-test was used to compare means. Results. Percent viability of cells exposed to liposomal bupivacaine for 30 minutes was less versus saline control (53.9% ± 21.5% vs. 73.7 ± 18.4%, p = 0.035), and this remained significant at 90 minutes (49.1% ± 20.3% vs. 67.2% ± 25.6%, p < 0.001). Liposomal bupivacaine had less chondrotoxic effects when compared with bupivacaine after 90 minutes, with greater viability (49.1% ± 20.3% vs. 21.4% ± 14.0%, p = 0.003). Chondrotoxicity was found to be time dependent within the bupivacaine group (percent viability at 30 min: 45.5 ± 18.2%, 90 min: 21.4 ± 14.0%, p = 0.001); however, liposomal bupivacaine did not demonstrate a significant timedependent chondrotoxic relationship (p = 0.583). Conclusions. Bupivacaine and liposomal bupivacaine are both toxic to chondrocytes. Liposomal bupivacaine is less chondrotoxic than standard bupivacaine and does not demonstrate a timedependent toxicity.https://www.hindawi.com/journals/aps/2020/5794187/ |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Travis Farmer S. Craig Morris Robert Quigley Nirav H. Amin Montri D. Wongworawat Hasan M. Syed |
spellingShingle |
Travis Farmer S. Craig Morris Robert Quigley Nirav H. Amin Montri D. Wongworawat Hasan M. Syed Chondrotoxicity of Local Anesthetics: Liposomal Bupivacaine Is Less Chondrotoxic than Standard Bupivacaine Advances in Pharmacological and Pharmaceutical Sciences |
author_facet |
Travis Farmer S. Craig Morris Robert Quigley Nirav H. Amin Montri D. Wongworawat Hasan M. Syed |
author_sort |
Travis Farmer |
title |
Chondrotoxicity of Local Anesthetics: Liposomal Bupivacaine Is Less Chondrotoxic than Standard Bupivacaine |
title_short |
Chondrotoxicity of Local Anesthetics: Liposomal Bupivacaine Is Less Chondrotoxic than Standard Bupivacaine |
title_full |
Chondrotoxicity of Local Anesthetics: Liposomal Bupivacaine Is Less Chondrotoxic than Standard Bupivacaine |
title_fullStr |
Chondrotoxicity of Local Anesthetics: Liposomal Bupivacaine Is Less Chondrotoxic than Standard Bupivacaine |
title_full_unstemmed |
Chondrotoxicity of Local Anesthetics: Liposomal Bupivacaine Is Less Chondrotoxic than Standard Bupivacaine |
title_sort |
chondrotoxicity of local anesthetics: liposomal bupivacaine is less chondrotoxic than standard bupivacaine |
publisher |
Hindawi Limited |
series |
Advances in Pharmacological and Pharmaceutical Sciences |
issn |
2633-4690 |
publishDate |
2020-01-01 |
description |
Objective. *e purpose of this study is to determine whether (1) liposomal bupivacaine is chondrotoxic; (2) the chondrotoxicity of
liposomal bupivacaine differs from standard bupivacaine; and (3) chondrotoxic effects are time dependent. Materials and
Methods. We obtained 72 10 mm articular cartilage plugs from 12 fresh bovine distal femoral knee joints and exposed them to
either saline, 0.5% bupivacaine, or liposomal bupivacaine for either 30 or 90 minutes. Twenty-four hours after treatment,
chondrocyte viability was measured with the use of a fluorescent live/dead assay. An ANOVA test of variance was performed
followed by a Holm–Sidak test to make pairwise comparisons across conditions. Student’s t-test was used to compare means.
Results. Percent viability of cells exposed to liposomal bupivacaine for 30 minutes was less versus saline control (53.9% ± 21.5% vs.
73.7 ± 18.4%, p = 0.035), and this remained significant at 90 minutes (49.1% ± 20.3% vs. 67.2% ± 25.6%, p < 0.001). Liposomal
bupivacaine had less chondrotoxic effects when compared with bupivacaine after 90 minutes, with greater viability (49.1% ± 20.3%
vs. 21.4% ± 14.0%, p = 0.003). Chondrotoxicity was found to be time dependent within the bupivacaine group (percent viability at
30 min: 45.5 ± 18.2%, 90 min: 21.4 ± 14.0%, p = 0.001); however, liposomal bupivacaine did not demonstrate a significant timedependent chondrotoxic relationship (p = 0.583). Conclusions. Bupivacaine and liposomal bupivacaine are both toxic to
chondrocytes. Liposomal bupivacaine is less chondrotoxic than standard bupivacaine and does not demonstrate a timedependent toxicity. |
url |
https://www.hindawi.com/journals/aps/2020/5794187/ |
work_keys_str_mv |
AT travisfarmer chondrotoxicityoflocalanestheticsliposomalbupivacaineislesschondrotoxicthanstandardbupivacaine AT scraigmorris chondrotoxicityoflocalanestheticsliposomalbupivacaineislesschondrotoxicthanstandardbupivacaine AT robertquigley chondrotoxicityoflocalanestheticsliposomalbupivacaineislesschondrotoxicthanstandardbupivacaine AT niravhamin chondrotoxicityoflocalanestheticsliposomalbupivacaineislesschondrotoxicthanstandardbupivacaine AT montridwongworawat chondrotoxicityoflocalanestheticsliposomalbupivacaineislesschondrotoxicthanstandardbupivacaine AT hasanmsyed chondrotoxicityoflocalanestheticsliposomalbupivacaineislesschondrotoxicthanstandardbupivacaine |
_version_ |
1715267344007168000 |