An improved nucleic acid extraction method from dried blood spots for amplification of Plasmodium falciparum kelch13 for detection of artemisinin resistance

Abstract Background Mutational analysis of the Plasmodium falciparum kelch 13 (k13) gene is routinely performed to track the emergence and spread of artemisinin resistance. Surveillance of resistance markers has been impeded by the difficulty of extracting sufficient DNA from low parasite density in...

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Bibliographic Details
Main Authors: Kayvan Zainabadi, Myaing M. Nyunt, Christopher V. Plowe
Format: Article
Language:English
Published: BMC 2019-06-01
Series:Malaria Journal
Subjects:
K13
Online Access:http://link.springer.com/article/10.1186/s12936-019-2817-8