Validation of picogram- and femtogram-input DNA libraries for microscale metagenomics

Validation of picogram- and femtogram-input DNA libraries for microscale metagenomics

High-throughput sequencing libraries are typically limited by the requirement for nanograms to micrograms of input DNA. This bottleneck impedes the microscale analysis of ecosystems and the exploration of low biomass samples. Current methods for amplifying environmental DNA to bypass this bottleneck...

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Bibliographic Details
Main Authors: Christian Rinke, Serene Low, Ben J. Woodcroft, Jean-Baptiste Raina, Adam Skarshewski, Xuyen H. Le, Margaret K. Butler, Roman Stocker, Justin Seymour, Gene W. Tyson, Philip Hugenholtz
Format: Article
Language:English
Published: PeerJ Inc. 2016-09-01
Series:PeerJ
Subjects:
Nextera XT
100 fg
Low input DNA library
Picogram
Reagent contamination
Low biomass
Online Access:https://peerj.com/articles/2486.pdf

Internet

https://peerj.com/articles/2486.pdf

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