Refined RIP-seq protocol for epitranscriptome analysis with low input materials.

N6-Methyladenosine (m6A) accounts for approximately 0.2% to 0.6% of all adenosine in mammalian mRNA, representing the most abundant internal mRNA modifications. m6A RNA immunoprecipitation followed by high-throughput sequencing (MeRIP-seq) is a powerful technique to map the m6A location transcriptom...

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Bibliographic Details
Main Authors: Yong Zeng, Shiyan Wang, Shanshan Gao, Fraser Soares, Musadeqque Ahmed, Haiyang Guo, Miranda Wang, Junjie Tony Hua, Jiansheng Guan, Michael F Moran, Ming Sound Tsao, Housheng Hansen He
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-09-01
Series:PLoS Biology
Online Access:http://europepmc.org/articles/PMC6136692?pdf=render