Forward Genetics by Sequencing EMS Variation-Induced Inbred Lines

In order to leverage novel sequencing techniques for cloning genes in eukaryotic organisms with complex genomes, the false positive rate of variant discovery must be controlled for by experimental design and informatics. We sequenced five lines from three pedigrees of ethyl methanesulfonate (EMS)-mu...

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Bibliographic Details
Main Authors: Charles Addo-Quaye, Elizabeth Buescher, Norman Best, Vijay Chaikam, Ivan Baxter, Brian P. Dilkes
Format: Article
Language:English
Published: Oxford University Press 2017-02-01
Series:G3: Genes, Genomes, Genetics
Subjects:
SNP
Online Access:http://g3journal.org/lookup/doi/10.1534/g3.116.029660