Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software
The median lethal dose (LD50) and low lethal dose (LD10) are calculated in acute toxicity studies, as well as during specific activity assessment of some medicines. The aim of the study was to develop a procedure for using CombiStats to calculate LD50 and LD10. The authors proposed a step-by-step al...
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OOO “Vashe Tsifrovoe Izdatelstvo”
2021-07-01
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Online Access: | https://www.vedomostincesmp.ru/jour/article/view/344 |
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doaj-45ae5520b30d449bb6d15a3c39549b742021-07-28T14:04:10ZrusOOO “Vashe Tsifrovoe Izdatelstvo”Ведомости Научного центра экспертизы средств медицинского применения1991-29192619-11722021-07-0111213514210.30895/1991-2919-2021-11-2-135-142265Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric SoftwareP. V. Shadrin0T. A. Batuashvili1L. V. Simutenko2N. P. Neugodova3Scientific Centre for Expert Evaluation of Medicinal ProductsScientific Centre for Expert Evaluation of Medicinal ProductsScientific Centre for Expert Evaluation of Medicinal ProductsScientific Centre for Expert Evaluation of Medicinal ProductsThe median lethal dose (LD50) and low lethal dose (LD10) are calculated in acute toxicity studies, as well as during specific activity assessment of some medicines. The aim of the study was to develop a procedure for using CombiStats to calculate LD50 and LD10. The authors proposed a step-by-step algorithm for processing bioassay results using the CombiStats biometric software (median effective dose determination model, probit analysis) with conversion of doses to simple fractions (fractions of the maximum dose) to calculate LD50 and LD10. They compared LD50 and LD10 calculation results obtained using CombiStats with those obtained using electronic spreadsheets according to the Bliss–Miller–Tainter–Prozorovsky method described in the State Pharmacopoeia of the Russian Federation (General Monograph 1.1.0014.15). It has been demonstrated that the use of CombiStats sometimes has advantages over the use of the pharmacoepoeial method.https://www.vedomostincesmp.ru/jour/article/view/344median lethal doseld<sub>50</sub>median effective doseed<sub>50</sub>low lethal doseld<sub>10</sub>combistatsbiometric softwaredose conversionmedicinal productssnake venom |
collection |
DOAJ |
language |
Russian |
format |
Article |
sources |
DOAJ |
author |
P. V. Shadrin T. A. Batuashvili L. V. Simutenko N. P. Neugodova |
spellingShingle |
P. V. Shadrin T. A. Batuashvili L. V. Simutenko N. P. Neugodova Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software Ведомости Научного центра экспертизы средств медицинского применения median lethal dose ld<sub>50</sub> median effective dose ed<sub>50</sub> low lethal dose ld<sub>10</sub> combistats biometric software dose conversion medicinal products snake venom |
author_facet |
P. V. Shadrin T. A. Batuashvili L. V. Simutenko N. P. Neugodova |
author_sort |
P. V. Shadrin |
title |
Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software |
title_short |
Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software |
title_full |
Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software |
title_fullStr |
Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software |
title_full_unstemmed |
Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software |
title_sort |
calculation of the median lethal dose and low lethal dose using the combistats biometric software |
publisher |
OOO “Vashe Tsifrovoe Izdatelstvo” |
series |
Ведомости Научного центра экспертизы средств медицинского применения |
issn |
1991-2919 2619-1172 |
publishDate |
2021-07-01 |
description |
The median lethal dose (LD50) and low lethal dose (LD10) are calculated in acute toxicity studies, as well as during specific activity assessment of some medicines. The aim of the study was to develop a procedure for using CombiStats to calculate LD50 and LD10. The authors proposed a step-by-step algorithm for processing bioassay results using the CombiStats biometric software (median effective dose determination model, probit analysis) with conversion of doses to simple fractions (fractions of the maximum dose) to calculate LD50 and LD10. They compared LD50 and LD10 calculation results obtained using CombiStats with those obtained using electronic spreadsheets according to the Bliss–Miller–Tainter–Prozorovsky method described in the State Pharmacopoeia of the Russian Federation (General Monograph 1.1.0014.15). It has been demonstrated that the use of CombiStats sometimes has advantages over the use of the pharmacoepoeial method. |
topic |
median lethal dose ld<sub>50</sub> median effective dose ed<sub>50</sub> low lethal dose ld<sub>10</sub> combistats biometric software dose conversion medicinal products snake venom |
url |
https://www.vedomostincesmp.ru/jour/article/view/344 |
work_keys_str_mv |
AT pvshadrin calculationofthemedianlethaldoseandlowlethaldoseusingthecombistatsbiometricsoftware AT tabatuashvili calculationofthemedianlethaldoseandlowlethaldoseusingthecombistatsbiometricsoftware AT lvsimutenko calculationofthemedianlethaldoseandlowlethaldoseusingthecombistatsbiometricsoftware AT npneugodova calculationofthemedianlethaldoseandlowlethaldoseusingthecombistatsbiometricsoftware |
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