Calcitriol in the Presence of Conditioned Media from Metastatic Breast Cancer Cells Enhances Ex Vivo Polarization of M2 Alternative Murine Bone Marrow-Derived Macrophages

Calcitriol in the Presence of Conditioned Media from Metastatic Breast Cancer Cells Enhances Ex Vivo Polarization of M2 Alternative Murine Bone Marrow-Derived Macrophages

In this study, we differentiated murine bone marrow-derived macrophages (BMDMs) into M0, M1, and M2 in the presence or absence of calcitriol. Real-time PCR analysis of gene expression, FACS analysis of surface markers, and chemokine/cytokine production assays were performed. In addition, the effect...

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Bibliographic Details
Main Authors: Artur Anisiewicz, Natalia Łabędź, Izabela Krauze, Joanna Wietrzyk
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Cancers
Subjects:
macrophages
BMDMs
vitamin D
calcitriol
breast cancer cells
M2 macrophages
Online Access:https://www.mdpi.com/2072-6694/12/11/3485

Internet

https://www.mdpi.com/2072-6694/12/11/3485

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