A standard for near-scarless plasmid construction using reusable DNA parts

Construction of plasmids from multiple fragments often uses customised parts and leaves scars where fragments are joined. Here the authors develop a method for barcoding fragments and constructing plasmids in a scarless manner from a collection of standard parts.

Bibliographic Details
Main Authors: Xiaoqiang Ma, Hong Liang, Xiaoyi Cui, Yurou Liu, Hongyuan Lu, Wenbo Ning, Nga Yu Poon, Benjamin Ho, Kang Zhou
Format: Article
Language:English
Published: Nature Publishing Group 2019-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-11263-0