A standard for near-scarless plasmid construction using reusable DNA parts
Construction of plasmids from multiple fragments often uses customised parts and leaves scars where fragments are joined. Here the authors develop a method for barcoding fragments and constructing plasmids in a scarless manner from a collection of standard parts.
Main Authors: | , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2019-07-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-019-11263-0 |