Soft substrates promote homogeneous self-renewal of embryonic stem cells via downregulating cell-matrix tractions.

Soft substrates promote homogeneous self-renewal of embryonic stem cells via downregulating cell-matrix tractions.

Maintaining undifferentiated mouse embryonic stem cell (mESC) culture has been a major challenge as mESCs cultured in Leukemia Inhibitory Factor (LIF) conditions exhibit spontaneous differentiation, fluctuating expression of pluripotency genes, and genes of specialized cells. Here we show that, in s...

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Bibliographic Details
Main Authors: Farhan Chowdhury, Yanzhen Li, Yeh-Chuin Poh, Tamaki Yokohama-Tamaki, Ning Wang, Tetsuya S Tanaka
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-12-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3001487?pdf=render

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http://europepmc.org/articles/PMC3001487?pdf=render

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