qPCR and loop mediated isothermal amplification for rapid detection of Ustilago tritici

Loose smut of wheat caused by the basidiomycete fungus Ustilago tritici, a seed-borne disease, is difficult to control because of the expanse of wheat planting area and difficulty in pathogen detection. In this study, real-time fluorescence quantitative PCR (qPCR) and loop-mediated isothermal amplif...

Full description

Bibliographic Details
Main Authors: Hanwen Yan, Jian Zhang, Dongfang Ma, Junliang Yin
Format: Article
Language:English
Published: PeerJ Inc. 2019-09-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/7766.pdf