Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis

Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis

Abstract Background Besides Escherichia coli, Bacillus subtilis is an important bacterial species for the production of recombinant proteins. Recombinant genes are inserted into shuttle expression vectors which replicate in both E. coli and in B. subtilis. The ligation products are first transformed...

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Bibliographic Details
Main Authors: Dinh Thi Minh Tran, Trang Thi Phuong Phan, Thanh Kieu Huynh, Ngan Thi Kim Dang, Phuong Thi Kim Huynh, Tri Minh Nguyen, Tuom Thi Tinh Truong, Thuoc Linh Tran, Wolfgang Schumann, Hoang Duc Nguyen
Format: Article
Language:English
Published: BMC 2017-07-01
Series:Microbial Cell Factories
Subjects:
Bacillus subtilis
Inducer-free expression vector
IPTG-inducible promoter
Pspac
Pgrac
Pgrac100
Online Access:http://link.springer.com/article/10.1186/s12934-017-0747-0

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http://link.springer.com/article/10.1186/s12934-017-0747-0

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