Establishment of in vitro 3D spheroid cell cultivation from human gynecologic cancer tissues

Establishment of in vitro 3D spheroid cell cultivation from human gynecologic cancer tissues

Summary: Advanced-stage gynecologic cancer remains a life-threatening disease. Here, we present a protocol for establishment of stable in vitro 3D spheroid cells derived from human uterine endometrial and ovarian cancer tissues. The tumor-derived spheroid cells have cancer stem cell-related characte...

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Bibliographic Details
Main Authors: Haruka Ueda, Yutaro Mori, Kaoru Yamawaki, Tatsuya Ishiguro, Hirokazu Ohata, Ai Sato, Kentaro Sugino, Nozomi Yachida, Manako Yamaguchi, Kazuaki Suda, Ryo Tamura, Kosuke Yoshihara, Koji Okamoto, Takayuki Enomoto
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Cell culture
Cancer
Stem cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000617

Internet

http://www.sciencedirect.com/science/article/pii/S2666166721000617

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