Using Precisely Defined in vivo Microbiotas to Understand Microbial Regulation of IgE
Early life exposure to microbes plays an important role in immune system development. Germ-free mice, or mice colonized with a low-diversity microbiota, exhibit high serum IgE levels. An increase in microbial richness, providing it occurs in a critical developmental window early in life, leads to in...
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doaj-466d0c87b5aa46ee83fef24ddec54f992020-11-25T00:12:55ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-01-011010.3389/fimmu.2019.03107479611Using Precisely Defined in vivo Microbiotas to Understand Microbial Regulation of IgEMadeleine Wyss0Kirsty Brown1Carolyn A. Thomson2Mia Koegler3Fernanda Terra4Vina Fan5Francesca Ronchi6Dominique Bihan7Ian Lewis8Markus B. Geuking9Kathy D. McCoy10Department of Physiology and Pharmacology, Cumming School of Medicine, Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB, CanadaDepartment of Physiology and Pharmacology, Cumming School of Medicine, Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB, CanadaDepartment of Physiology and Pharmacology, Cumming School of Medicine, Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB, CanadaDepartment of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB, CanadaDepartment of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB, CanadaDepartment of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB, CanadaDepartment of Biomedical Research, University of Bern, Bern, SwitzerlandDepartment of Biological Sciences, University of Calgary, Calgary, AB, CanadaDepartment of Biological Sciences, University of Calgary, Calgary, AB, CanadaDepartment of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB, CanadaDepartment of Physiology and Pharmacology, Cumming School of Medicine, Snyder Institute for Chronic Diseases, University of Calgary, Calgary, AB, CanadaEarly life exposure to microbes plays an important role in immune system development. Germ-free mice, or mice colonized with a low-diversity microbiota, exhibit high serum IgE levels. An increase in microbial richness, providing it occurs in a critical developmental window early in life, leads to inhibition of this hygiene-induced IgE. However, whether this inhibition is dependent solely on certain microbial species, or is an additive effect of microbial richness, remains to be determined. Here we report that mice colonized with a combination of bacterial species with specific characteristics is required to inhibit IgE levels. These defined characteristics include the presence in early life, acetate production and immunogenicity reflected by induction of IgA. Suppression of IgE did not correlate with production of the short chain fatty acids propionate and butyrate, or induction of peripherally induced Tregs in mucosal tissues. Thus, inhibition of IgE induction can be mediated by specific microbes and their associated metabolic pathways and immunogenic properties.https://www.frontiersin.org/article/10.3389/fimmu.2019.03107/fullmicrobiotaIgETregsSCFAgnotobiotic |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Madeleine Wyss Kirsty Brown Carolyn A. Thomson Mia Koegler Fernanda Terra Vina Fan Francesca Ronchi Dominique Bihan Ian Lewis Markus B. Geuking Kathy D. McCoy |
spellingShingle |
Madeleine Wyss Kirsty Brown Carolyn A. Thomson Mia Koegler Fernanda Terra Vina Fan Francesca Ronchi Dominique Bihan Ian Lewis Markus B. Geuking Kathy D. McCoy Using Precisely Defined in vivo Microbiotas to Understand Microbial Regulation of IgE Frontiers in Immunology microbiota IgE Tregs SCFA gnotobiotic |
author_facet |
Madeleine Wyss Kirsty Brown Carolyn A. Thomson Mia Koegler Fernanda Terra Vina Fan Francesca Ronchi Dominique Bihan Ian Lewis Markus B. Geuking Kathy D. McCoy |
author_sort |
Madeleine Wyss |
title |
Using Precisely Defined in vivo Microbiotas to Understand Microbial Regulation of IgE |
title_short |
Using Precisely Defined in vivo Microbiotas to Understand Microbial Regulation of IgE |
title_full |
Using Precisely Defined in vivo Microbiotas to Understand Microbial Regulation of IgE |
title_fullStr |
Using Precisely Defined in vivo Microbiotas to Understand Microbial Regulation of IgE |
title_full_unstemmed |
Using Precisely Defined in vivo Microbiotas to Understand Microbial Regulation of IgE |
title_sort |
using precisely defined in vivo microbiotas to understand microbial regulation of ige |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2020-01-01 |
description |
Early life exposure to microbes plays an important role in immune system development. Germ-free mice, or mice colonized with a low-diversity microbiota, exhibit high serum IgE levels. An increase in microbial richness, providing it occurs in a critical developmental window early in life, leads to inhibition of this hygiene-induced IgE. However, whether this inhibition is dependent solely on certain microbial species, or is an additive effect of microbial richness, remains to be determined. Here we report that mice colonized with a combination of bacterial species with specific characteristics is required to inhibit IgE levels. These defined characteristics include the presence in early life, acetate production and immunogenicity reflected by induction of IgA. Suppression of IgE did not correlate with production of the short chain fatty acids propionate and butyrate, or induction of peripherally induced Tregs in mucosal tissues. Thus, inhibition of IgE induction can be mediated by specific microbes and their associated metabolic pathways and immunogenic properties. |
topic |
microbiota IgE Tregs SCFA gnotobiotic |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2019.03107/full |
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