Induction of Tertiary Lymphoid Structures With Antitumor Function by a Lymph Node-Derived Stromal Cell Line
Tertiary lymphoid structures (TLSs) associate with better prognosis in certain cancer types, but their underlying formation and immunological benefit remain to be determined. We established a mouse model of TLSs to study their contribution to antitumor immunity. Because the stroma in lymph nodes (sL...
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doaj-46cd8b619d2d498ab2902f6713f81d7d2020-11-24T22:10:53ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-07-01910.3389/fimmu.2018.01609376553Induction of Tertiary Lymphoid Structures With Antitumor Function by a Lymph Node-Derived Stromal Cell LineGenyuan Zhu0Satoshi Nemoto1Adam W. Mailloux2Patricio Perez-Villarroel3Ryosuke Nakagawa4Rana Falahat5Anders E. Berglund6James J. Mulé7James J. Mulé8Immunology Program, Moffitt Cancer Center, Tampa, FL, United StatesImmunology Program, Moffitt Cancer Center, Tampa, FL, United StatesImmunology Program, Moffitt Cancer Center, Tampa, FL, United StatesImmunology Program, Moffitt Cancer Center, Tampa, FL, United StatesImmunology Program, Moffitt Cancer Center, Tampa, FL, United StatesImmunology Program, Moffitt Cancer Center, Tampa, FL, United StatesDepartment of Biostatistics and Bioinformatics, Moffitt Cancer Center, Tampa, FL, United StatesImmunology Program, Moffitt Cancer Center, Tampa, FL, United StatesCutaneous Oncology Program, Moffitt Cancer Center, Tampa, FL, United StatesTertiary lymphoid structures (TLSs) associate with better prognosis in certain cancer types, but their underlying formation and immunological benefit remain to be determined. We established a mouse model of TLSs to study their contribution to antitumor immunity. Because the stroma in lymph nodes (sLN) participates in architectural support, lymphogenesis, and lymphocyte recruitment, we hypothesized that TLSs can be created by sLN. We selected a sLN line with fibroblast morphology that expressed sLN surface markers and lymphoid chemokines. The subcutaneous injection of the sLN line successfully induced TLSs that attracted infiltration of host immune cell subsets. Injection of MC38 tumor lysate-pulsed dendritic cells activated TLS-residing lymphocytes to demonstrate specific cytotoxicity. The presence of TLSs suppressed MC38 tumor growth in vivo by improving antitumor activity of tumor-infiltrating lymphocytes with downregulated immune checkpoint proteins (PD-1 and Tim-3). Future engineering of sLN lines may allow for further enhancements of TLS functions and immune cell compositions.https://www.frontiersin.org/article/10.3389/fimmu.2018.01609/fulltertiary lymphoid structuresstromal cellslymphogenesistumor-infiltrating lymphocytesimmune checkpoint proteins |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Genyuan Zhu Satoshi Nemoto Adam W. Mailloux Patricio Perez-Villarroel Ryosuke Nakagawa Rana Falahat Anders E. Berglund James J. Mulé James J. Mulé |
spellingShingle |
Genyuan Zhu Satoshi Nemoto Adam W. Mailloux Patricio Perez-Villarroel Ryosuke Nakagawa Rana Falahat Anders E. Berglund James J. Mulé James J. Mulé Induction of Tertiary Lymphoid Structures With Antitumor Function by a Lymph Node-Derived Stromal Cell Line Frontiers in Immunology tertiary lymphoid structures stromal cells lymphogenesis tumor-infiltrating lymphocytes immune checkpoint proteins |
author_facet |
Genyuan Zhu Satoshi Nemoto Adam W. Mailloux Patricio Perez-Villarroel Ryosuke Nakagawa Rana Falahat Anders E. Berglund James J. Mulé James J. Mulé |
author_sort |
Genyuan Zhu |
title |
Induction of Tertiary Lymphoid Structures With Antitumor Function by a Lymph Node-Derived Stromal Cell Line |
title_short |
Induction of Tertiary Lymphoid Structures With Antitumor Function by a Lymph Node-Derived Stromal Cell Line |
title_full |
Induction of Tertiary Lymphoid Structures With Antitumor Function by a Lymph Node-Derived Stromal Cell Line |
title_fullStr |
Induction of Tertiary Lymphoid Structures With Antitumor Function by a Lymph Node-Derived Stromal Cell Line |
title_full_unstemmed |
Induction of Tertiary Lymphoid Structures With Antitumor Function by a Lymph Node-Derived Stromal Cell Line |
title_sort |
induction of tertiary lymphoid structures with antitumor function by a lymph node-derived stromal cell line |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2018-07-01 |
description |
Tertiary lymphoid structures (TLSs) associate with better prognosis in certain cancer types, but their underlying formation and immunological benefit remain to be determined. We established a mouse model of TLSs to study their contribution to antitumor immunity. Because the stroma in lymph nodes (sLN) participates in architectural support, lymphogenesis, and lymphocyte recruitment, we hypothesized that TLSs can be created by sLN. We selected a sLN line with fibroblast morphology that expressed sLN surface markers and lymphoid chemokines. The subcutaneous injection of the sLN line successfully induced TLSs that attracted infiltration of host immune cell subsets. Injection of MC38 tumor lysate-pulsed dendritic cells activated TLS-residing lymphocytes to demonstrate specific cytotoxicity. The presence of TLSs suppressed MC38 tumor growth in vivo by improving antitumor activity of tumor-infiltrating lymphocytes with downregulated immune checkpoint proteins (PD-1 and Tim-3). Future engineering of sLN lines may allow for further enhancements of TLS functions and immune cell compositions. |
topic |
tertiary lymphoid structures stromal cells lymphogenesis tumor-infiltrating lymphocytes immune checkpoint proteins |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2018.01609/full |
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