tPA promotes ADAMTS-4-induced CSPG degradation, thereby enhancing neuroplasticity following spinal cord injury
Although tissue plasminogen activator (tPA) is known to promote neuronal remodeling in the CNS, no mechanism of how this plastic function takes place has been reported so far. We provide here in vitro and in vivo demonstrations that this serine protease neutralizes inhibitory chondroitin sulfate pro...
Main Authors: | , , , , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2014-06-01
|
Series: | Neurobiology of Disease |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996114000473 |
id |
doaj-46d1b00f83784e43a3e73f6c7be4158e |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sighild Lemarchant Mathilde Pruvost Marie Hébert Maxime Gauberti Yannick Hommet Aurélien Briens Eric Maubert Yatma Gueye François Féron Didier Petite Marcel Mersel Jean-Claude do Rego Hubert Vaudry Jari Koistinaho Carine Ali Véronique Agin Evelyne Emery Denis Vivien |
spellingShingle |
Sighild Lemarchant Mathilde Pruvost Marie Hébert Maxime Gauberti Yannick Hommet Aurélien Briens Eric Maubert Yatma Gueye François Féron Didier Petite Marcel Mersel Jean-Claude do Rego Hubert Vaudry Jari Koistinaho Carine Ali Véronique Agin Evelyne Emery Denis Vivien tPA promotes ADAMTS-4-induced CSPG degradation, thereby enhancing neuroplasticity following spinal cord injury Neurobiology of Disease Type 4 disintegrin and metalloproteinase with thrombospondin motifs Tissue plasminogen activator Spinal cord injury Chondroitin sulfate proteoglycans Neurocan Neuroplasticity |
author_facet |
Sighild Lemarchant Mathilde Pruvost Marie Hébert Maxime Gauberti Yannick Hommet Aurélien Briens Eric Maubert Yatma Gueye François Féron Didier Petite Marcel Mersel Jean-Claude do Rego Hubert Vaudry Jari Koistinaho Carine Ali Véronique Agin Evelyne Emery Denis Vivien |
author_sort |
Sighild Lemarchant |
title |
tPA promotes ADAMTS-4-induced CSPG degradation, thereby enhancing neuroplasticity following spinal cord injury |
title_short |
tPA promotes ADAMTS-4-induced CSPG degradation, thereby enhancing neuroplasticity following spinal cord injury |
title_full |
tPA promotes ADAMTS-4-induced CSPG degradation, thereby enhancing neuroplasticity following spinal cord injury |
title_fullStr |
tPA promotes ADAMTS-4-induced CSPG degradation, thereby enhancing neuroplasticity following spinal cord injury |
title_full_unstemmed |
tPA promotes ADAMTS-4-induced CSPG degradation, thereby enhancing neuroplasticity following spinal cord injury |
title_sort |
tpa promotes adamts-4-induced cspg degradation, thereby enhancing neuroplasticity following spinal cord injury |
publisher |
Elsevier |
series |
Neurobiology of Disease |
issn |
1095-953X |
publishDate |
2014-06-01 |
description |
Although tissue plasminogen activator (tPA) is known to promote neuronal remodeling in the CNS, no mechanism of how this plastic function takes place has been reported so far. We provide here in vitro and in vivo demonstrations that this serine protease neutralizes inhibitory chondroitin sulfate proteoglycans (CSPGs) by promoting their degradation via the direct activation of endogenous type 4 disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS-4). Accordingly, in a model of compression-induced spinal cord injury (SCI) in rats, we found that administration of either tPA or its downstream effector ADAMTS-4 restores the tPA-dependent activity lost after the SCI and thereby, reduces content of CSPGs in the spinal cord, a cascade of events leading to an improved axonal regeneration/sprouting and eventually long term functional recovery. This is the first study to reveal a tPA–ADAMTS-4 axis and its function in the CNS. It also raises the prospect of exploiting such cooperation as a therapeutic tool for enhancing recovery after acute CNS injuries. |
topic |
Type 4 disintegrin and metalloproteinase with thrombospondin motifs Tissue plasminogen activator Spinal cord injury Chondroitin sulfate proteoglycans Neurocan Neuroplasticity |
url |
http://www.sciencedirect.com/science/article/pii/S0969996114000473 |
work_keys_str_mv |
AT sighildlemarchant tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT mathildepruvost tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT mariehebert tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT maximegauberti tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT yannickhommet tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT aurelienbriens tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT ericmaubert tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT yatmagueye tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT francoisferon tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT didierpetite tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT marcelmersel tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT jeanclaudedorego tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT hubertvaudry tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT jarikoistinaho tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT carineali tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT veroniqueagin tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT evelyneemery tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury AT denisvivien tpapromotesadamts4inducedcspgdegradationtherebyenhancingneuroplasticityfollowingspinalcordinjury |
_version_ |
1724208337853087744 |
spelling |
doaj-46d1b00f83784e43a3e73f6c7be4158e2021-03-22T12:40:59ZengElsevierNeurobiology of Disease1095-953X2014-06-01662842tPA promotes ADAMTS-4-induced CSPG degradation, thereby enhancing neuroplasticity following spinal cord injurySighild Lemarchant0Mathilde Pruvost1Marie Hébert2Maxime Gauberti3Yannick Hommet4Aurélien Briens5Eric Maubert6Yatma Gueye7François Féron8Didier Petite9Marcel Mersel10Jean-Claude do Rego11Hubert Vaudry12Jari Koistinaho13Carine Ali14Véronique Agin15Evelyne Emery16Denis Vivien17Inserm UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular Unit, University of Caen Basse-Normandie, GIP CYCERON, F-14074 Caen Cedex, FranceInserm UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular Unit, University of Caen Basse-Normandie, GIP CYCERON, F-14074 Caen Cedex, FranceInserm UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular Unit, University of Caen Basse-Normandie, GIP CYCERON, F-14074 Caen Cedex, FranceInserm UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular Unit, University of Caen Basse-Normandie, GIP CYCERON, F-14074 Caen Cedex, FranceInserm UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular Unit, University of Caen Basse-Normandie, GIP CYCERON, F-14074 Caen Cedex, FranceInserm UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular Unit, University of Caen Basse-Normandie, GIP CYCERON, F-14074 Caen Cedex, FranceInserm UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular Unit, University of Caen Basse-Normandie, GIP CYCERON, F-14074 Caen Cedex, FranceCNRS UMR-6184, Neurobiologie des Interactions Cellulaires et Neurophysiopathologie, IFR Jean Roche, Faculté de Médecine, University of Aix-Marseille, F-13916 Marseille, FranceCNRS UMR-6184, Neurobiologie des Interactions Cellulaires et Neurophysiopathologie, IFR Jean Roche, Faculté de Médecine, University of Aix-Marseille, F-13916 Marseille, FranceInserm UMR-S 583, Institute for Neurosciences of Montpellier, Pathophysiology and Therapy of Sensory and Motor Deficits, Saint Eloi Hospital, F-34091 Montpellier, FranceInserm UMR-S 583, Institute for Neurosciences of Montpellier, Pathophysiology and Therapy of Sensory and Motor Deficits, Saint Eloi Hospital, F-34091 Montpellier, FranceInserm UMR-S 982, Différenciation et Communication Neuronale et Neuroendocrine, PRIMACEN, IFRMP 23, University of Rouen, F-76130 Mont-Saint-Aignan, FranceInserm UMR-S 982, Différenciation et Communication Neuronale et Neuroendocrine, PRIMACEN, IFRMP 23, University of Rouen, F-76130 Mont-Saint-Aignan, FranceDepartment of Neurobiology, A. I. Virtanen Institute for Molecular Sciences, Biocenter Kuopio, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio, FinlandInserm UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular Unit, University of Caen Basse-Normandie, GIP CYCERON, F-14074 Caen Cedex, FranceInserm UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular Unit, University of Caen Basse-Normandie, GIP CYCERON, F-14074 Caen Cedex, FranceInserm UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular Unit, University of Caen Basse-Normandie, GIP CYCERON, F-14074 Caen Cedex, France; Department of Neurosurgery, Caen University Hospital, Avenue de la Côte de Nacre, F-14000 Caen, France; Corresponding authors at: Inserm UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular Unit, University of Caen Basse-Normandie, GIP CYCERON, Bd Becquerel, BP 5229, F-14074 Caen Cedex, France. Fax: +33 2 31470222.Inserm UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular Unit, University of Caen Basse-Normandie, GIP CYCERON, F-14074 Caen Cedex, France; Corresponding authors at: Inserm UMR-S 919, Serine Proteases and Pathophysiology of the Neurovascular Unit, University of Caen Basse-Normandie, GIP CYCERON, Bd Becquerel, BP 5229, F-14074 Caen Cedex, France. Fax: +33 2 31470222.Although tissue plasminogen activator (tPA) is known to promote neuronal remodeling in the CNS, no mechanism of how this plastic function takes place has been reported so far. We provide here in vitro and in vivo demonstrations that this serine protease neutralizes inhibitory chondroitin sulfate proteoglycans (CSPGs) by promoting their degradation via the direct activation of endogenous type 4 disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS-4). Accordingly, in a model of compression-induced spinal cord injury (SCI) in rats, we found that administration of either tPA or its downstream effector ADAMTS-4 restores the tPA-dependent activity lost after the SCI and thereby, reduces content of CSPGs in the spinal cord, a cascade of events leading to an improved axonal regeneration/sprouting and eventually long term functional recovery. This is the first study to reveal a tPA–ADAMTS-4 axis and its function in the CNS. It also raises the prospect of exploiting such cooperation as a therapeutic tool for enhancing recovery after acute CNS injuries.http://www.sciencedirect.com/science/article/pii/S0969996114000473Type 4 disintegrin and metalloproteinase with thrombospondin motifsTissue plasminogen activatorSpinal cord injuryChondroitin sulfate proteoglycansNeurocanNeuroplasticity |