An Apical Meristem-Targeted in planta Transformation Method for the Development of Transgenics in Flax (Linum usitatissimum): Optimization and Validation

An Apical Meristem-Targeted in planta Transformation Method for the Development of Transgenics in Flax (Linum usitatissimum): Optimization and Validation

Efficient regeneration of explants devoid of intrinsic somaclonal variations is a cardinal step in plant tissue culture, thus, a vital component of transgenic technology. However, recalcitrance of economically important crops to tissue culture-based organogenesis ensues a setback in the use of trans...

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Bibliographic Details
Main Authors: Karthik Kesiraju, Shaily Tyagi, Soumyadeep Mukherjee, Rhitu Rai, Nagendra K. Singh, Rohini Sreevathsa, Prasanta K. Dash
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-01-01
Series:Frontiers in Plant Science
Subjects:
transgenic flax/linseed
in planta transformation
apical meristem
GUS
nptII
GM crops
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2020.562056/full

Internet

https://www.frontiersin.org/articles/10.3389/fpls.2020.562056/full

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