Collagenase Lot Selection and Purification for Adipose Tissue Digestion
Crude Clostridial collagenase (CCC) remains the most widely used enzyme for the digestion of tissues prior to cell isolation and culture. CCC contains numerous components in addition to specific collagenases and proteases. A chronic problem associated with CCC is significant lot variability which oc...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
SAGE Publishing
1995-05-01
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Series: | Cell Transplantation |
Online Access: | https://doi.org/10.1177/096368979500400306 |